Catalog #SIM0102FAB
InVivoSIM anti-human TNFα (Certolizumab Biosimilar)
Clone
Certolizumab
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This biosimilar Certolizumab antibody uses the same variable regions as the therapeutic antibody Certolizumab, making it ideal for research use. Certolizumab is an antigen-binding fragment (Fab′) that specifically targets tumor necrosis factor alpha (TNF-α), a central pro-inflammatory cytokine involved in autoimmune and inflammatory diseases. Unlike full-length IgG antibodies, Certolizumab lacks an Fc region and therefore does not engage Fcγ receptors or activate complement, enabling selective neutralization of soluble and membrane-associated TNF-α without Fc-mediated effector functions such as ADCC or CDC. While the therapeutic form of Certolizumab is PEGylated to extend serum half-life, this research-use biosimilar is not PEGylated, allowing investigators to study TNF-α neutralization independent of PEG-related pharmacokinetic or biological effects. This Certolizumab biosimilar is well suited for investigating TNF-α biology, inflammatory signaling pathways, and for benchmarking the therapeutic antibody’s target engagement and neutralizing activity in pre-clinical experimental systems where Fc-mediated functions are not desired.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human TNFα |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation* |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 50 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |