InVivoSIM anti-human Tissue Factor (CD142) (Tisotumab Biosimilar)
Catalog #SIM0084
Clone:
Tisotumab
Reactivities:
Human, Monkey
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Tisotumab, making it ideal for research use. Tisotumab is a humanized IgG1 monoclonal antibody that reacts with human and monkey tissue factor (TF), also known as CD142, coagulation factor III (F3), and thromboplastin. TF is a single-pass transmembrane glycoprotein that is essential for initiating blood coagulation by forming a complex with circulating factor VII (F7) or serum prothrombin conversion accelerator (SPCA). This interaction converts the factor VII to VIIa, which follows the TF:VIIa complex-mediated activation of factors IX or X through proteolysis. Recent research has explored the involvement of CD142 in additional cellular signaling pathways responsible for tumor growth, e.g., mitogen-activated protein kinase (MAPK), phosphatidylinositol-3-kinase AKT (PI3K-AKT), and Wnt signaling pathways. TF shows aberrant expression in some tumors, and owing to its internalizing capacity, an in vivo study with patient-derived xenograft models of solid tumors has explored the potential of a monomethyl auristatin E (MMAE)-based antibody-drug conjugate (ADC) targeting TF for its anti-cancer activity. The tisotumab biosimilar is an antibody portion of an ADC called tisotumab vedotin, which is known for targeting the TF antigen, thereby directly delivering the cytotoxic microtubule-disrupting agent MMAE to cancer cells.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R |
| Immunogen | Human Tissue Factor (CD142) |
| Reported Applications |
Antibody-drug conjugate synthesis In vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer