Catalog #SIMB0109
InVivoSIM anti-human PD-1 x CTLA-4 (Vudalimab Biosimilar)
Clone
Vudalimab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
in vitro functional assays
ELISA
Isotype
Human IgG1, κ
Product Description
This Vudalimab biosimilar antibody uses the same variable regions as the therapeutic antibody Vudalimab, making it ideal for research use. Vudalimab is a humanized IgG1-based bispecific antibody that simultaneously targets human programmed cell death protein 1 (PD-1; PDCD1; CD279) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4; CD152), two inhibitory immune checkpoint receptors that regulate T-cell activation and antitumor immunity. The molecule combines an anti-PD-1 IgG arm with an anti-CTLA-4 single-chain variable fragment (scFv) to enable dual checkpoint targeting within a single molecule. By simultaneously engaging PD-1 and CTLA-4, Vudalimab inhibits two complementary mechanisms of T-cell suppression and is designed to preferentially target T cells co-expressing both checkpoints, including tumor-infiltrating lymphocytes. Dual checkpoint blockade can restore T-cell activation, proliferation, and cytotoxic immune responses within the tumor microenvironment. This Vudalimab biosimilar is well suited for studying PD-1 and CTLA-4 biology, T-cell exhaustion, dual checkpoint blockade, tumor-infiltrating lymphocyte function, and for benchmarking the therapeutic antibody's mechanism of action in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E233P/L234V/L235A/G236del/S267K/E357Q/S364K/L368D/K370S/M428L/N434S |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |