InVivoSIM anti-human PD-1 (Sintilimab Biosimilar)
Catalog #SIM0054
Clone:
Sintilimab
Reactivities:
Human, Monkey
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Sintilimab, making it ideal for research use. Sintilimab is a humanized IgG4 kappa monoclonal antibody with an S228P hinge stabilization mutation that specifically reacts with programmed cell death protein 1 (PD-1), also known as CD279. Sintilimab is shown to have a greater affinity for human PD-1 compared to both nivolumab and pembrolizumab. Sintilimab also shows cross-reactivity to PD-1 cynomolgus monkey species. PD-1 is an inhibitory receptor belonging to the CD28 family of the Ig superfamily, and it is transiently expressed on the cell surface of CD4 and CD8 T cells, B cells, as well as myeloid cells. PD-L1 is the main ligand of PD-1 and is expressed on the surface of tumor cells and antigen-presenting cells (APCs). PD-1 and PD-L1 interactions are central to immune homeostasis and prevention of autoimmunity. PD-1 binding to PD-L1 inhibits T cell activation, thereby diminishing T cell proliferation, cytokine production, and cytotoxic activity. This phenomenon is critical in the downregulation of the immune response during chronic inflammation and minimizing tissue damage. Cancers, such as squamous cell carcinoma, lung cancer, melanoma, ovarian cancer, renal cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma, often express PD-L1 in high abundance, wherein the cancer cells exploit the PD1/PD-L1 pathway to evade immune surveillance via immune evasion. In immune checkpoint inhibitor-responsive experimental models, the tumor growth can be transiently arrested in vivo through treatment with anti-PD-1 antibodies, e.g., sintilimab, which block the interactions between PD-1 and PD-L1 proteins.Specifications
| Isotype | Human IgG4, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S228P |
| Immunogen | Human PD-1 |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer