InVivoSIM anti-human IL‑3RA (CD123) (Pivekimab Biosimilar)
Catalog #SIM0080
Clone:
Pivekimab
Reactivities:
Human
Product Details
This biosimilar antibody uses the same variable regions as the therapeutic antibody Pivekimab, making it ideal for research use. Pivekimab is a humanized IgG1 monoclonal antibody that specifically targets CD123, also known as interleukin‑3 receptor alpha (IL‑3RA). CD123 is highly expressed on malignant hematopoietic cells, including acute myeloid leukemia (AML) and blastic plasmacytoid dendritic cell neoplasm (BPDCN), and plays a critical role in IL‑3–mediated cell survival and proliferation. By binding to CD123, Pivekimab blocks IL‑3 signaling pathways involved in the pathogenesis of myeloid malignancies. While the therapeutic version (pivekimab sunirine) is conjugated to a cytotoxic payload, this Pivekimab biosimilar is unconjugated and intended for research use only. It is well suited for studying IL‑3 signaling, CD123 expression, and receptor blockade in hematologic cancers.Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L/S442C/KDel |
| Immunogen | Human IL‑3RA |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer