InVivoSIM anti-human IL-33 (Etokimab Biosimilar)
Catalog #SIM0053
Clone:
Etokimab
Reactivities:
Human, Monkey
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Etokimab, making it ideal for research use. Etokimab is a humanized IgG1 monoclonal antibody that reacts with interleukin-33 (IL-33) of human and cynomolgus monkey species. IL-33 is a member of the IL-1 family of cytokines, and it is constitutively expressed by epithelial cells, endothelial cells, and fibroblastic stromal cells. IL-33 is an alarmin cytokine which is released when a cell or tissue is damaged. IL-33 plays an important role in tissue homeostasis/repair, type 2 immunity, viral infection, allergic and non-allergic inflammation, and cancer. The binding of secreted IL-33 to its receptor ST2, also called the IL-1 receptor accessory protein (IL-1RAP) heterodimer, triggers MyD88-dependent NF-ĪŗB or AP-1 signaling pathways, thereby upregulating inflammatory cytokines crucial to the pathogenesis of both allergic and non-allergic inflammatory disorders. IL-33 targets Th1 cells, Th2 cells, basophils, eosinophils, macrophages, DCs, neutrophils, CD8+ T cells, NK cells, and iNKT cells. The etokimab antibody (formerly known as ANB020) has been shown to inhibit the release of IFN-Ī³ by IL-33/IL-12 in in vitro preclinical experiments. Etokimab has been used to neutralize IL-33 in various diseases, including atopic dermatitis (AD), eosinophilic asthma, and chronic rhinosinusitis with nasal polyps (CRSwNP), as well as allergic inflammation in various tissues (such as skin and nasopharyngeal/pulmonary tissues).Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K214R |
| Immunogen | Human IL-33 |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
-
Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer