InVivoSIM anti-human IL-31RA (Nemolizumab Biosimilar)
Catalog #SIM0078
Clone:
Nemolizumab
Reactivities:
Human
Product Details
This nonātherapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Nemolizumab making it ideal for research use. Nemolizumab is a humanized IgG2 monoclonal antibody that specifically targets the interleukin-31 receptor alpha (IL-31RA). ILā31RA is primarily expressed on keratinocytes, dorsal root ganglion (DRG) neurons, monocytes, macrophages, and dendritic cells, where it mediates the biological effects of ILā31, a cytokine known for driving pruritus, inflammation, and epithelial barrier dysfunction. By binding to IL-31RA, it blocks the signaling of interleukin-31 (ILā31)āa neuroimmune cytokine that drives itch (pruritus), skin inflammation, barrier dysfunction, and fibrosisāthereby interrupting the pathogenic itchāscratch cycle seen in skin disorders like atopic dermatitis (AD) and prurigo nodularis (PN)āÆ. Nemolizumab has been shown to provide rapid and sustained relief of pruritus in clinical trials involving AD and PN by modulating neuroimmune pathways, improving skin integrity, and reducing inflammationāÆ. This Nemolizumab biosimilar is well suited for studying ILā31 signaling, neuroimmune interactions in itch, barrier function, and the development of anti-pruritic therapeutics.Specifications
| Isotype | Human IgG2, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | C130S/R132K/E136G/S137G/C218S/ H263Q/R350Q/Q414E/G441DEL/K442DEL |
| Immunogen | Human IL-31RA |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer