InVivoSIM anti-human IGF-1R (Teprotumumab Biosimilar)
Catalog #SIM0070
Clone:
Teprotumumab
Product Details
This nonātherapeutic biosimilar antibody adopts the same variable regions as the therapeutic antibody Teprotumumab, making it ideal for research use. Teprotumumab is a fully human IgG1 monoclonal antibody that targets the insulinālike growth factorā1 receptor (IGFā1R), a tyrosine kinase receptor broadly expressed across numerous cell types and prominently upregulated in thyroid eye disease (TED). In orbital fibroblasts, IGFā1R forms functional complexes with the TSH receptor and drives pathogenic signaling cascades that lead to inflammation, tissue expansion, and remodeling in the orbit. IGFā1R is activated by IGFā1, a growth factor critical for regulating cell growth and tissue development both prenatally and postnatally. IGFā1 promotes proliferation, migration, and differentiation in a wide variety of cell types and has broad metabolic functions, including regulation of glucose and lipid metabolism, modulation of steroid hormone signaling, and maintenance of cartilage and bone homeostasis. It also contributes to skeletal muscle regeneration and has been implicated in tumor progression and survival signaling in cancer. Teprotumumab binds the extracellular Ī±-subunit of IGFā1R, blocking receptor activation and downstream signaling via the PI3K/AKT and MAPK/ERK pathways, thus dampening inflammatory responses and reducing hyaluronan production and tissue remodeling.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human IGF-1R |
| Reported Applications |
Functional assays Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (LALA-PG) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer