InVivoSIM anti-human FGFR2b (CD332) (Bemarituzumab Biosimilar)
Catalog #SIM0052
Clone:
Bemarituzumab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody, Bemarituzumab, making it ideal for research use. Bemarituzumab is a humanized IgG1Īŗ anti-human FGFR2b (CD332) antibody, which reacts with the fibroblast growth factor receptor 2 IIIb isoform (FGFR2b) of FGFR2. FGFR2 is a member of a family of four FGF receptors that interact with over 20 different fibroblast growth factors (FGFs), each of which shows relatively unique and restricted expression to specific tissues, cell types, and/or developmental stages. FGF7, FGF10, and FGF22 are the main ligands of FGFR2b. FGFR2b is expressed in epithelial tissues such as the stomach and skin and is often found overexpressed in gastric cancers. FGF-FGFR2 signaling is involved in many cancers, such as gastric, breast, ovarian, endometrial, lung, and bile duct cancers. Bemarituzumab has recently been introduced for the therapeutic blockade of FGFR2b signaling. In combination with anti-cancer experimental therapeutics, bemarituzumab is documented to exhibit substantial synergism in vivo with immune checkpoint blockage via anti-PD-1 or chemotherapy.NOTE: The CHOK1BN-Fut8KO cell line was not used to express this bemarituzumab antibody (SIM0052), so it is NOT afucosylated.
Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Mutations | K214R |
| Immunogen | Human FGFR2b |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer