InVivoSIM anti-human FGF‑23 (Burosumab Biosimilar)
Catalog #SIM0079
Clone:
Burosumab
Product Details
This biosimilar antibody uses the same variable regions as the therapeutic antibody Burosumab making it ideal for research use. Burosumab is a fully human IgG1 monoclonal antibody that specifically targets fibroblast growth factor 23 (FGF‑23). It binds circulating FGF‑23, blocking its interaction with the FGFR1–α‑Klotho receptor complex, thereby restoring phosphate homeostasis by enhancing renal phosphate reabsorption and increasing active vitamin D production. FGF‑23 is a hormone secreted by osteocytes and osteoblasts that regulates serum phosphate levels by inhibiting phosphate reabsorption in the kidney and suppressing vitamin D synthesis, ultimately reducing intestinal phosphate absorption. Clinically, Burosumab is approved for the treatment of X‑linked hypophosphatemia (XLH) and tumor-induced osteomalacia (TIO) in both adults and children. It has demonstrated significant improvements in serum phosphate, bone mineralization, rickets severity, and growth in XLH patients, with sustained efficacy and safety over extended use. This Burosumab biosimilar is well suited for studying phosphate and vitamin D metabolism, endocrine FGF signaling, bone biology, and therapeutic blockade of FGF‑23.Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | E356D/M358L |
| Immunogen | Human FGF-23 |
| Reported Applications |
in vivo functional assays in vitro functional assays Flow cytometry ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer