Catalog #SIM0026
InVivoSIM anti-human Claudin 18.2 (Zolbetuximab Biosimilar)
Clone
Zolbetuximab
Reactivities
Mouse, Human, Monkey
Applications
Functional assays
Isotype
Human IgG1, κ
Product Description
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Zolbetuximab making it ideal for research use. This Zolbetuximab biosimilar antibody reacts with isoform 2 of human claudin 18. Claudin 18.2, a member of the claudin family, is commonly expressed in multiple cancers, including gastric and pancreatic cancer. Zolbetuximab (developmental name: IMAB362) is reported to cross-react with monkey and mouse Claudin 18.2 also. Claudins play a major role in tight junctions, which establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They also play an important role in tumorigenesis and inflammation. Alterations in claudin expression have been shown to lead to impaired functions of tight junctions, influence signaling pathways, and act as tumor-promoting events in some epithelial cancers. Claudin 18.2 is one of its important isoforms and is mainly expressed in the stomach (gastric mucosa) and is absent from other healthy tissues. In a humanized mouse model of prostate cancer Zolbetuximab demonstrated antitumor activity by stimulating ADCC and/or CDC.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human claudin 18.2 |
| Reported Applications |
Functional assays |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_3696365 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |