Catalog #SIM0120
InVivoSIM anti-human CGRP (Fremanezumab biosimilar)
Clone
Fremanezumab
Reactivities
Human
Isotype
Human IgG2, κ
Product Description
This Fremanezumab biosimilar antibody uses the same variable regions as the therapeutic antibody Fremanezumab, making it ideal for research use. Fremanezumab is a humanized IgG2a monoclonal antibody that specifically targets calcitonin gene-related peptide (CGRP), a neuropeptide that plays a central role in migraine pathophysiology. CGRP is released from sensory neurons within the trigeminovascular system and contributes to vasodilation, neurogenic inflammation, and pain transmission associated with migraine attacks. By binding soluble CGRP with high affinity, Fremanezumab prevents its interaction with the calcitonin gene-related peptide receptor, thereby inhibiting downstream signaling involved in migraine initiation and propagation. In addition to migraine, CGRP plays important roles in nociception, vascular homeostasis, and neurogenic inflammation, making it an important target for studying neurovascular biology. This Fremanezumab biosimilar is well suited for studying CGRP biology, neurovascular signaling, pain mechanisms, migraine pathophysiology, and for benchmarking the therapeutic antibody's target engagement and neutralizing activity in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG2, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG2 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | A326S/P327S |
| Immunogen | Human CGRP |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |