Catalog #SIM0101
InVivoSIM anti-human CGRP (Eptinezumab biosimilar)
Clone
Eptinezumab
Reactivities
Human
Isotype
Human IgG1, κ
Product Description
This Eptinezumab biosimilar antibody uses the same variable regions as the therapeutic antibody Eptinezumab, making it ideal for research use. Eptinezumab is a humanized IgG1 monoclonal antibody that specifically targets calcitonin gene-related peptide (CGRP), a neuropeptide that plays a central role in migraine pathophysiology. CGRP is widely expressed in sensory neurons of the trigeminovascular system and is a potent vasodilator and mediator of neurogenic inflammation. Elevated CGRP levels are associated with migraine attacks, where it contributes to vasodilation, nociceptive signaling, and central sensitization. By binding soluble CGRP with high affinity, Eptinezumab prevents CGRP from engaging its receptor complex (CLR/RAMP1), thereby inhibiting downstream signaling pathways involved in migraine initiation and propagation. Clinically, Eptinezumab has demonstrated efficacy in reducing migraine frequency and severity in patients with episodic and chronic migraine. This Eptinezumab biosimilar is well suited for studying CGRP biology, neurovascular signaling, neurogenic inflammation, pain pathways, and for benchmarking the therapeutic antibody’s target engagement and neutralizing activity in pre-clinical experimental systems.
Specifications
| Isotype | Human IgG1, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (N297A) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | K213A/K214R/F241T/N297A |
| Immunogen | Human CGRP |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |