InVivoSIM anti-human CD79b (Polatuzumab Biosimilar)
Catalog #SIM0060
Clone:
Polatuzumab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Polatuzumab, making it ideal for research use. Polatuzumab is a humanized IgG1 monoclonal antibody that targets CD79b, a critical component of the B cell receptor (BCR) complex. CD79b, also known as IgĪ², forms a heterodimer with CD79a (IgĪ±) and is essential for signal transduction following antigen engagement of surface immunoglobulin. The CD79a/CD79b complex initiates downstream signaling cascades that regulate B cell development, activation, proliferation, and survival. CD79b is expressed throughout most stages of B cell maturation but is absent from plasma cells and non-B lineage cells. Importantly, CD79b is retained on the surface of many malignant B cells, including those in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma, making it an attractive therapeutic and diagnostic target. Polatuzumab serves as the antibody backbone for the FDA-approved antibody-drug conjugate Polatuzumab vedotin, which delivers a cytotoxic MMAE payload to CD79b+ cells. In contrast, this unconjugated biosimilar form lacks any drug conjugation, allowing researchers to study CD79b-specific antibody binding, internalization, and function without the confounding effects of cytotoxicity. This Polatuzumab biosimilar antibody retains the antigen-binding domains of the therapeutic but is produced for non-clinical research use. It is suitable for a range of applications, including flow cytometry, B cell targeting studies, preclinical modeling, and ADC development.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Human CD79b |
| Reported Applications |
Functional assays Flow cytometry Immunoprecipitation ELISA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer