Catalog #SIM0105
InVivoSIM anti-human CD28 (Theralizumab biosimilar)
Clone
Theralizumab
Reactivities
Human
Applications
in vivo functional assays
in vitro functional assays
ELISA
Flow cytometry
in vitro functional assays
ELISA
Flow cytometry
Isotype
Human IgG4 (S228P)
Product Description
This biosimilar antibody uses the same variable regions as the therapeutic antibody Theralizumab, making it ideal for research use. Theralizumab (TGN1412) is a humanized IgG4 monoclonal antibody that targets CD28, a co-stimulatory receptor expressed on T cells. CD28 provides essential signals for T cell activation, proliferation, and cytokine production upon engagement with its ligands CD80 and CD86 on antigen-presenting cells. Theralizumab is a superagonist antibody, meaning it can activate T cells independently of T cell receptor (TCR) signaling, leading to robust polyclonal T cell activation and cytokine release. This mechanism highlighted the critical role of CD28 in immune regulation, as well as the risks associated with uncontrolled immune activation. This Theralizumab biosimilar is well suited for studying T cell co-stimulation, immune activation, and cytokine release mechanisms in vitro and in vivo.
Specifications
| Isotype | Human IgG4 (S228P) - Kappa |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S228P |
| Immunogen | Human CD28 |
| Reported Applications |
in vivo functional assays in vitro functional assays ELISA Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |