InVivoSIM anti-human CD27 (Varlilumab Biosimilar)
Catalog #SIM0087
Clone:
Varlilumab
Reactivities:
Human
Product Details
This nonātherapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Varlilumab, making it ideal for research use. Varlilumab is a human IgG1 monoclonal antibody that specifically targets CD27, a member of the tumor necrosis factor receptor (TNFR) superfamily. CD27 is a co-stimulatory immune receptor that is constitutively expressed on naive and memory T cells, and can also be found on regulatory T cells (Tregs), NK cells, and some B cells. Engagement of CD27 by its ligand, CD70, promotes T cell activation, survival, and effector function, playing a key role in regulating immune responses and maintaining long-term immunity. Varlilumab is an agonist antibody that mimics CD70 binding, delivering potent co-stimulatory signals to T cells while also depleting CD27āŗ regulatory T cells via Fc-mediated effector mechanisms. Varlilumab has shown therapeutic potential in enhancing anti-tumor immunity in cancer models and has been investigated in combination with immune checkpoint inhibitors such as anti-PD-1. This Varlilumab biosimilar is ideal for studying CD27 signaling, T cell costimulation, Treg modulation, and combination immunotherapy mechanisms in both in vitro and in vivo settings.Specifications
| Isotype | Human IgG4, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S228P |
| Immunogen | Human CD27 |
| Reported Applications |
In vivo functional assays in vitro functional assays Flow cytometry Western blot ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer