Catalog #SIM0087
InVivoSIM anti-human CD27 (Varlilumab Biosimilar)
Clone
Varlilumab
Reactivities
Human
Applications
In vivo functional assays
in vitro functional assays
Flow cytometry
Western blot
ELISA
in vitro functional assays
Flow cytometry
Western blot
ELISA
Isotype
Human IgG4, κ
Product Description
This non‑therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Varlilumab, making it ideal for research use. Varlilumab is a human IgG1 monoclonal antibody that specifically targets CD27, a member of the tumor necrosis factor receptor (TNFR) superfamily. CD27 is a co-stimulatory immune receptor that is constitutively expressed on naive and memory T cells, and can also be found on regulatory T cells (Tregs), NK cells, and some B cells. Engagement of CD27 by its ligand, CD70, promotes T cell activation, survival, and effector function, playing a key role in regulating immune responses and maintaining long-term immunity. Varlilumab is an agonist antibody that mimics CD70 binding, delivering potent co-stimulatory signals to T cells while also depleting CD27⁺ regulatory T cells via Fc-mediated effector mechanisms. Varlilumab has shown therapeutic potential in enhancing anti-tumor immunity in cancer models and has been investigated in combination with immune checkpoint inhibitors such as anti-PD-1. This Varlilumab biosimilar is ideal for studying CD27 signaling, T cell costimulation, Treg modulation, and combination immunotherapy mechanisms in both in vitro and in vivo settings.
Specifications
| Isotype | Human IgG4, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S228P |
| Immunogen | Human CD27 |
| Reported Applications |
In vivo functional assays in vitro functional assays Flow cytometry Western blot ELISA |
| Formulation |
pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |