Catalog #BE0485
InVivoMAb anti-mouse CXCR5 (CD185)
Clone
Cx5Mab-3
Reactivities
Mouse
Isotype
Rat IgG2b, κ
Product Description
The Cx5Mab-3 monoclonal antibody reacts with mouse C-X-C chemokine receptor type 5 (CXCR5), also known as Gpcr6, Blr1, and CD185. The CXCR5 protein is mainly expressed as a cell surface receptor on B cells and a subset of T cells (e.g., T follicular helper or Tfh cells). CXCR5’s primary ligand is CXCL13 that is also known as B-cell attracting chemokine 1 (BLC). Upon activation, CXCR5 couples with heterotrimeric G proteins to activate downstream PI3K/Akt, PLC, Rac and MAPK ignaling pathways. The CXCR5-CXCL13 signaling is essential for B-cell homing to follicles and for Tfh cell migration in lymphoid organs, facilitating immune responses. The interaction of CXCR5+ Tfh cells with germinal center B cells drives their differentiation into plasma cells or memory B cell formation during infection, autoimmunity, and cancer. CXCR5 is expressed in cancers also, and high PD-1-expressing CD8+ T cells in tumors overexpress CXCL13. The CXCL13-CXCR5 axis-mediated upregulation of lymphocyte infiltration, activation, and differentiation enhances the antitumor immune responses. Like CD8+ T effector cells, the CXCR5+ CD8+ T cells also depict cytolytic activity in tumor microenvironments. CXCR5 as well as its ligand CXCL13 are emerging as attractive targets/biomarkers for immune checkpoint inhibition immunotherapy. NOTE – please see Ishikawa et. al. Microbes & Immunity. 2025; 2(1): 101-113 (doi: 10.36922/mi.5664) for the original characterization data of the Cx5Mab-3 antibody.
Specifications
| Isotype | Rat IgG2b, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | A synthetic peptide corresponding to the N-terminal sequence of mouse CXCR5 (MNYPLTLDMGSITYNMDDL) |
| Reported Applications |
Flow cytometry ELISA For details on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |