Catalog #BE0507FAB2
InVivoMAb anti-mouse CD5 f(ab')2 fragments
Clone
53-7.3 f(ab')2 Fragments
Reactivities
Mouse
Isotype
Rat IgG2a, κ
Product Description
This product is an F(ab′)₂ fragment derived from the anti-mouse CD5 antibody clone 53-7.3 (also known as 53-7.313). CD5 is a 65 kDa lymphoid-specific glycoprotein expressed on T cells and a subset of B cells known as B1a cells. It plays a role in the regulation of T cell receptor (TCR) and B cell receptor (BCR) signaling, thymocyte selection, T cell differentiation, and immune tolerance. Unlike a full-length antibody, this F(ab′)₂ fragment contains two antigen-binding regions linked by disulfide bonds and lacks the Fc domain. This format retains specific binding to CD5 while eliminating Fc-mediated effector functions such as Fcγ receptor engagement and Fc-dependent complement activation. Because F(ab′)₂ fragments are bivalent, they retain the ability to cross-link CD5 and may influence receptor signaling. This format is useful in experiments where CD5 targeting or receptor engagement is desired without Fc-mediated immune interactions or depletion. Anti-CD5 F(ab′)₂ fragments may also be explored as targeting components for nanoparticles or other delivery systems directed toward CD5-expressing cells. This anti-mouse CD5 F(ab′)₂ fragment therefore provides a useful reagent for studies of CD5 receptor engagement, lymphocyte signaling, and targeted delivery approaches where Fc-mediated effects are undesirable.
Specifications
| Isotype | Rat IgG2a, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse Spleen Cells |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Pepsin Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |