FlowMAb FITC anti-mouse/human CD44
Catalog #FM0039-FITC
Clone:
IM7
Reactivities:
Mouse, Human
Product Details
The IM7 monoclonal antibody reacts with human and mouse CD44, also known as Hermes, HCAM, and Pgp-1. CD44 is an 80-95 kDa glycoprotein that is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As an adhesion molecule, CD44 participates in a wide variety of cellular functions, including lymphocyte activation, recirculation and homing, and hematopoiesis. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Moreover, tumor metastasis involves CD44, and antibodies targeting CD44 have demonstrated a reduction in the malignant activities of various neoplasms. Interestingly, high levels of the adhesion molecule CD44 on leukemic cells are essential to generating leukemia. The IM7 antibody (also known as IM7.8.1) is often used as an anti-pan CD44 marker antibody in experiments because it recognizes a highly conserved epitope outside the HA-binding domain of all isoforms of the CD44 core protein. This fluorescein isothiocyanate (FITC)-conjugated version of the IM7antibody is useful for flow cytometry, immunofluorescence, immunohistochemistry (frozen), and immunohistochemistry (paraffin) applications.Specifications
| Isotype | Rat IgG2b,Ā Īŗ |
|---|---|
| Recommended Isotype Control(s) | FlowMAb FITC rat IgG2b isotype control, anti-keyhole limpet hemocyanin |
| Conjugation | FITC |
| Excitation Source | Blue 488 nm |
| Excitation Max | 494 nm |
| Emission Max | 518 nm |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells |
| Formulation |
PBS, pH 7.0 Contains 0.09% Sodium Azide |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G. Conjugated with fluorescein isothiocyanate under optimal conditions. |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C and protected from prolonged exposure to light. Do not freeze. |
Additional Formats
Recommended Products
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Recommended Isotype Control(s)
FlowMAb FITC rat IgG2b isotype control, anti-keyhole limpet hemocyanin
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer