Catalog #CPB532
Bispecific anti-mouse TIGIT x anti-mouse CD25 (IL-2Rα) - PREORDER
Clone
derived from clones 1G9 and 7D4
Reactivities
Mouse
Isotype
mouse IgG2a, κ
Product Description
CPB532 is a 1+1 bivalent bispecific designed to simultaneously target mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT) and mouse CD25 (IL-2Rα). CPB532 contains the murine IgG2a constant region to reduce immunogenicity and the formation of anti-drug antibodies (ADAs) in mouse models. This bispecific was designed to selectively deplete intratumoral Tregs that co-express both CD25 and TIGIT, while sparing most peripheral Tregs and activated effector T cells creating a stronger anti-tumor immune response. CD25 targeting helps identify activated, highly suppressive Tregs in the tumor microenvironment, where CD25 expression is enriched on the most immunosuppressive subset. TIGIT targeting adds tumor selectivity because TIGIT is also enriched on tumor Tregs and marks a suppressive, activated phenotype. Anti-TIGIT x anti-CD25 bispecifics work by co-targeting a double-positive intratumoral Treg population to selectively remove suppressive cells and restore anti-tumor immunity.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Reported Applications |
ELISA For information on in-vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 145.4 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |