Catalog #CPB545
Bispecific anti-mouse PD-1 x anti-mouse VEGF-A (LALA-PG) - PREORDER
Clone
derived from clones RMP1-14 and B20-4.1.1
Reactivities
Mouse, Human
Applications
ELISA
For information on in vivo applications, please contact technicalservice@bioxcell.com
For information on in vivo applications, please contact technicalservice@bioxcell.com
Isotype
Mouse IgG2a (LALA-PG)-scFv, κ
Product Description
CPB545 is a 2+2 symmetric tetravalent bispecific engineered to simultaneously target mouse programmed cell death protein 1 (PD-1, CD279) and mouse or human vascular endothelial growth factor A (VEGF-A) proteins. CPB545 contains the murine IgG2a constant region to reduce immunogenicity and the formation of anti-drug antibodies (ADAs) in mouse models and LALA-PG Fc-silencing mutations to abolish antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) while minimizing Fc-driven off-target toxicity. This bispecific was designed to concurrently block two key pathways involved in tumor progression and immune evasion through dual PD-1 and VEGF-A inhibition. The tetravalent structure provides four antigen-binding sites to enable cooperative binding that can leverage target proximity within the tumor microenvironment.
Specifications
| Isotype | mouse IgG2a (LALA-PG)-scFv, κ |
|---|---|
| Recommended Isotype Control(s) | Bispecific mouse IgG2a (LALA-PG)-scFv, kappa isotype control |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Reported Applications |
ELISA For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Molecular Weight | 201.3 |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |