Catalog #CPB514
Bispecific anti-mouse PD-L1 x anti-mouse VEGF - PREORDER
Clone
Derived from clones 2G11-2A05 and 10F.9G2
Reactivities
Mouse
Isotype
mouse IgG2a (LALA-PG)-scFv, κ
Product Description
CPB514 is a 2+2 symmetric tetravalent bispecific engineered to simultaneously target mouse programmed death-ligand 1 (PD-L1, CD274, B7-H1) and mouse vascular endothelial growth factor A (VEGF-A), integrating checkpoint inhibition and anti-angiogenesis into a single molecule. CPB514 contains the murine IgG2a constant region to reduce immunogenicity and the formation of anti-drug antibodies (ADAs) in mouse models and LALA-PG Fc-silencing mutations to abolish antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) while minimizing Fc-driven off-target toxicity and focusing the mechanism on checkpoint/angiogenesis blockade rather than target-cell depletion. The PD-L1 arm binds tumor and myeloid PD-L1 to prevent interaction with PD-1 on T cells, restoring TCR signaling, NFAT activation, proliferation and cytotoxic function of effector T cells. The VEGF arm sequesters VEGF-A, preventing engagement of VEGFR1/2 on endothelial cells and inhibits downstream PI3K–AKT–mTOR and related pathways that drive angiogenesis, vascular permeability and tumor growth. Relieving PD-1/PD-L1–mediated immunosuppression and blocking VEGF-driven angiogenesis with cooperative binding can enrich bispecific molecules within VEGF/PD-L1-rich tumor microenvironments. This dual targeting can localize anti-VEGF activity to PD-L1–expressing tumor sites while enhancing T-cell activation and vascular normalization.
Specifications
| Isotype | mouse IgG2a (LALA-PG)-scFv, κ |
|---|---|
| Recommended Isotype Control(s) | Bispecific mouse IgG2a (LALA-PG)-scFv, kappa isotype control |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Reported Applications |
ELISA For information on in-vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Molecular Weight | 200.2 |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |