Catalog #CPB504
Bispecific anti-mouse PD-1 x anti-mouse CTLA-4 - PREORDER
Clone
Derived from clones 9D9 and RMP1-14
Reactivities
Mouse
Isotype
mouse IgG2a (LALA-PG), κ
Product Description
CPB504 is a 1+1 bivalent bispecific checkpoint inhibitor antibody that targets both mouse programmed cell death protein‑1 (PD‑1, CD279) and mouse cytotoxic T‑lymphocyte–associated antigen‑4 (CTLA-4) to enhance T‑cell–mediated antitumor immunity. CPB504 contains the murine IgG2a constant region to reduce immunogenicity and the formation of anti-drug antibodies (ADAs) in mouse models and LALA-PG Fc-silencing mutations to abolish antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) while minimizing Fc-driven off-target toxicity. This bispecific is designed to simultaneously bind PD-1 and CTLA-4 on T cells, particularly tumor‑infiltrating lymphocytes, to engage both checkpoints on the same or neighboring T cells and provide coordinated dual checkpoint blockade within the tumor microenvironment. Blocking PD‑1 prevents interaction with PD‑L1/PD‑L2 and reverses PD‑1–mediated inhibitory signaling, restoring T‑cell activation, proliferation, and effector function against tumor cells. Concurrent CTLA‑4 blockade reduces competition with CD28 for CD80/CD86, enhancing costimulatory signaling and promoting expansion and priming of effector T cells, further amplifying antitumor responses.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | Bispecific mouse IgG2a (LALA-PG), kappa isotype control |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Reported Applications |
ELISA For information on in-vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 136.3 kD |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |